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Title 

Characterization of 1,925 Bacillus thuringiensis isolates from plants in Korea

 

국내 식물시료에서 분리한 Bacillus thuringiensis 균주의 다양성

Authors 

Seung Hwan ParkBon Tag KooByung Sik ShinSoo Keun ChoiYoung-Mee JeongJae Gu PanJeong Il Kim

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1997

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 25, no. 2, pp. 159-165

Keywords 

Agelastica coeruleaBacillus thuringiensisInsecticidal activityPhylloplaneScreening

Abstract 

We collected 3,237 plant samples, mainly leaves of various trees, from many provinces in Korea and a total of 1,925 Bacillus thuringiensis isolates were obtained and characterized. The isolates were characterized in terms of crystal morphology, PAGE pattern of the toxin proteins, plasmids pattern, biochemical characteristics, and bioassay. The microscopic observation showed that 49.1% of the isolates have bipyramidal shape crystals, 7.1% of spherical shape crystals, 1.4% of rhomboidal shape crystals, and others have small or amorphous inclusions. The insecticidal activities of the spore-crystal mixtures of isolates were tested against Plutella xylostella, Bombyx mori, Culex pipiens, and Agelastica coerulea. Bioassay showed that 51.3% of the isolates were shown to be active; lepidopteran-specific (44.8%), dipteran-specific(4.9%) and coleopteran-specific (1.6%). The remainder(48.8%) did not show any activity against the insects we tested. Interestingly though, some of these non-active isolates were shown to have bipyramidal crystals. By serotyping 22 isolates of our collection, we found that there are various kind of subspecies such as aizawai, amagiens, canadensis, darmstadiensis, galleriae, finitimus, kurstaki, morrisoni and neoleonensis, and three isolates have been classified into a new serotype, H49, and one of them, the type strain, named subsp. muju. From this study it was found that phylloplane is a good source for the isolation of Bacillus thuringiensis, and Bacillus thuringiensis is distributed widely in Korea.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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