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Title 

Novel polyethylene glycol/levan aqueous two-phase system for protein partitioning

Authors 

Bong Hyun ChungWon Kyung KimKi Bang SongChul Ho KimSang Ki Rhee

Publisher 

Springer

Issue Date 

1997

Citation 

Biotechnology Techniques, vol. 11, no. 5, pp. 327-329

Abstract 

A novel aqueous two-phase system was made by mixing PEG (polyethylene glycol) and levan. The partitioning experiments were performed with six model proteins: horse heart cytochrome c, horse hemoglobin, horse heart myoglobin, hen egg albumin, bovine serum albumin and hen egg lysozyme. The addition of NaCl increased the partition coefficient of lysozyme but decreased that of albumin. The partition coefficients were sensitive to pH, increasing in the pH range of 6 to 8 for five of the proteins tested and decreasing slightly for lysozyme.

ISSN 

0951-208X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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