상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Wild-type p53 triggers a rapid senescence program in human tumor cells lacking functional p53

Authors 

M M SugrueDeug Y ShinSam W LeeS A Aaronson

Publisher 

National Academy of Sciences

Issue Date 

1997

Citation 

Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 18, pp. 9648-9653

Keywords 

EJTumor suppressioncyclin adna fragmentprotein p53rnabladder carcinomacancer inhibitioncarcinoma celldna transfection

Abstract 

The p53 tumor suppressor gene has been shown to play an important role in determining cell fate. Overexpression of wild-type p53 in tumor cells has been shown to lead to growth arrest or apoptosis. Previous studies in fibroblasts have provided indirect evidence for a link between p53 and senescence. Here we show, using an inducible p53 expression system, that wild-type p53 overexpression in EJ bladder carcinoma cells, which have lost functional p53, triggers the rapid onset of G1 and G2/M growth arrest associated with p21 upregulation and repression of mitotic cyclins (cyclin A and B) and cdc2. Growth arrest in response to p53 induction became irreversible within 48-72 h, with cells exhibiting morphological features as well as specific biochemical and ultrastructural markers of the senescent phenotype. These findings provide direct evidence that p53 overexpression can activate the rapid onset of senescence in tumor cells.

ISSN 

0027-8424

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)