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Title 

Genetic transformation of Streptomyces caespitosus

Authors 

Jin Cheol YooJung-Bo SimSung-Jun KimSi-Wouk KimJung Joon Lee

Publisher 

Pharmaceutical Society of Korea

Issue Date 

1993

Citation 

Archives of Pharmacal Research, vol. 16, no. 4, pp. 300-304

Keywords 

genetic transformationstreptomyces

Abstract 

Genetic transformation of Streptomyces caespitosus by plasmid pIJ 702 was carried out. Optimal conditions for the protoplast preparation of Streptomyces caespitosus, its regeneration, and its transformation by pIJ 702 were evaluated. Addition of 2% glycine to the culture broth was optimal for protoplast yield. Formation and regeneration of protoplasts were most efficient when the mycelium were harvested at between late log and stationary growth phase. The regeneration frequency of the protoplasts was 15% when the protoplasts were regenerated on R2YE agar media containing 0.5M sucrose. Under the best condition for protoplate-regeneration, the optimal transformation frequency was achieved with 40% polyethylene glycol (M.W. 4,000) treatment for 2 minutes.

ISSN 

0253-6269

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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