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Title 

Induction of differentiation in the cultured F9 teratocarcinoma stem cells by triterpene acids

Authors 

Ho-Young LeeHae-Young ChungKi-Heun KimJung Joon LeeKyu-Won Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

1994

Citation 

Journal of Cancer Research and Clinical Oncology, vol. 120, no. 0, pp. 513-518

Keywords 

DifferentiationF9 teratocarcinomaOleanolic acidUrsolic acidoleanolic acidretinoic acidretinoic acid binding proteintriterpeneursolic acidanimal cell

Abstract 

The effects of the triterpene acids, ursolic acid and oleanolic acid, on the differentiation of F9 teratocarcinoma stem cells were studied. These agents caused the morphological change of F9 cells into endoderm cells, as did retinoic acid (RA). Moreover, expression of laminin B1, type IV collagen and retinoic acid receptor β (RARβ) increased in ursolic- and oleanolic-acid treated F9 cells. Since these agents are structurally similar to the glucocorticoid hormone, we studied the effects of dexamethasone, a synthetic glucocorticoid, on F9 cells. Dexamethasone also induced the morphological change and altered the expression of larminin B1, type IV collagen, and RARβ in F9 cells. In addition, transcription of glucocorticoid receptor was detected after treatment with these three agents. According to Southwestern blot analysis, a 94-kDa protein, thought to be a glucocorticoid receptor, was detected in F9 cells treated with these agents. In a gel-shift assay, we identified protein factors binding to the glucocorticoid-responsive element (GRE) in the nuclear proteins from F9 cells treated with ursolic or oleanolic acid. The binding activity of the GRE-binding protein disappeared on the addition of unlabeled GRE oligonucleotide. Taken together, these results suggest that UA and OA can induce the differentiation of F9 cells and may regulate the expression of differentiation-specific genes, probably by forming a complex with the glucocorticoid receptor or its analogous nuclear receptor.

ISSN 

0171-5216

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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