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Title 

Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae

Authors 

Chi-Min ChoiMyoung-Dong KimSang Ki RheeJin-Ho Seo

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Biotechnology Letters, vol. 18, no. 10, pp. 1129-1132

Keywords 

hirudinrecombinant dnaculture mediumsaccharomyces cerevisiaeSaccharomycesSaccharomyces cerevisiae

Abstract 

The enriched medium based on yeast nitrogen base(YNB)increased hirudin synthesis and secretion in rccombinant Saccharomyces cerevisiae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudin/1 but supplementation of yeast extract increased the final hirudin concentration to 46lmg hirudin/1. The defined medium, however, produced the product protein with higher purity of 21% and hence will allow easy separation of secreted hirudin from other contaminated polypeptides present in the growth medium. In a continuous culuture, the defined medium yielded higher concentrations of cell mass and hirudin than the complex medium.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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