상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Identification of human liver cDNA clones whose products interact with G-protein β subunit of Schizosccharomyces pombe

Authors 

Mi Sun WonKyung-Mi MoonYoung Joo JangNam-Kyu SunDong Uk KimMi Young HanChoong Eun LeeKyung Bin SongHyang Sook Yoo

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Molecules and Cells, vol. 6, no. 6, pp. 645-652

Abstract 

Heterotrimeric GTP binding proteins (G-proteins) are composed of α, β, and γ subunits and have important roles in the cell signalling from cell surface receptors to effectors. The a subunit as well as the βγ complex are known to interact with effector molecules to deliver specific signals to downstream genes. In an attempt to identify the molecules interacting directly with G-protein β subunit (Gβ) in signal pathway, G-protein β subunit gene of Schizosaccharomyces pombe was used as bait to screen a human liver cDNA library in a yeast two-hybrid system. When the coding region DNA of the S. pombe β subunit gene, gpb1+, was cloned into the 3′-end of the GAL4 DNA binding domain sequence and used to screen a human liver cDNA library cloned at the 3′-end of the GAL4 activation domain, 42 novel clones showing β-galactosidase activity were obtained from the 9×106 transformants. The sequence analysis of these clones revealed that the four clones contain sequence homologies with a receptor tyrosine kinase, a mammalian homolog of hsp40 (DNAJ), a NMDA receptor glutamate-binding protein, and a protein serine kinase. These four clones showed specific binding to G-protein β subunit of S. pombe in vitro. The characterization of these proteins in the G-protein-mediated signal pathway will be useful in elucidating the precise mechanism of G-protein function.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)