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Title 

EcoRI variant N199H has enhanced specific activity

Authors 

Jae Jong KimKyung Tai MinMyung Hee KimS J AughByung-Dong KimDae Sil Lee

Publisher 

Elsevier

Issue Date 

1996

Citation 

Gene, vol. 171, no. 0, pp. 129-130

Keywords 

Site-directed mutagenesisenzyme activityprotein variantstructure activity relationDeoxyribonuclease EcoRI

Abstract 

The Asn199 residue of the EcoRI restriction endonuclease has been replaced with other amino acids to investigate whether it mediates nucleotide recognition or catalytic activity. Cassette mutagenesis gave variants of EcoRI: N199D, N199H, N199L, N199R, N199S and N199V. Their relative cleavage rates were found to be in the following order: N199H > EcoRI (wild type; wt) > N199L > N199V > N199S > N199R > N199D. In particular, EcoRI variant N199H showed about a twofold higher specific activity than that of the wt enzyme.

ISSN 

0378-1119

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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