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Title 

Cloning and sequencing of the PYRG encoding cytidine 5'-triphosphate synthetase from Mycobacterium bovis BCG

Authors 

Jeong Hyun KimJin Koo KimYong Kyung Choe

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1997

Citation 

Biochemistry and Molecular Biology International, vol. 43, no. 5, pp. 925-933

Keywords 

BCGCTP synthetaseGlutamine amidotransferase type IpyrGcytidine triphosphatesynthetasemolecular cloningmycobacterium bovisCloning, MolecularMycobacterium bovis

Abstract 

The pyrG gene encoding cytidine 5'-triphosphate (CTP) synthetase which catalyzes the terminal reaction for de novo synthesis of pyrimidine nucleotides was cloned and sequenced from Mycobacterium bovis BCG. The BCG pyrG was screened in λZAPII library employing a DNA probe which was constructed from PCR based on a consensus pattern of glutamine amidotransferase type I. The BCG CTP synthetase deduced from a nucleotide sequence consisting of 586 amino acid residues with a calculated molecular weight of 63,642 daltons. Alignment of BCG CTP synthetase with all sequences available in GenBank indicates that the residues suggested as a catalytic triad for glutamine hydrolysis at the carboxyl terminal domain and an amino terminal segment are completely conserved among all CTP synthetases.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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