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Title 

Complete sequence of the UPP gene encoding uracil phosphoribosyltransferase from Mycobacterium bovis BCG

Authors 

Jin Koo KimJeong Hyun KimSeung-Hwan LeeYoung KangJong-Seok LimHee Gu LeeSang Jae KimGill Han BaiYoung Kil ParkSang-Hyun ChoSang Won KangYu Sam KimIn Seong ChoeTai Wha ChungSue Nie ParkSeung Ho KimJong Seog AhnYong Kyung Choe

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1997

Citation 

Biochemistry and Molecular Biology International, vol. 41, no. 6, pp. 1117-1124

Keywords 

BCGuppUracil phosphoribosyltraltsferasebcg vaccineuracil phosphoribosyltransferaseamino acid sequencegenemycobacterium bovisnucleotide sequenceAmino Acid Sequence

Abstract 

The nucleotide sequence of a 799 bp fragment of Mycobacterium bovis BCG containing the putative upp gene that encodes a uracil phosphoribosyltransferase (UPRTase [EC 2.4.2.9]) has been determined. The upp gene of BCG has an open reading frame (ORF) of 621 bp (207 amino acids) starting with GTG (position 112) and ending with TGA (position 733), and its molecular mass was calculated to be 21,864 Da. Comparative analyses of the deduced amino acid sequence of BCG UPRTase with the UPRTase of six bacterial genera revealed that 24% (50/211) of die residues are perfectly conserved and 32% (67/211) of the residues are well conserved.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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