상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Molecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis

Authors 

Mi Young HanMi Young SonSeung-Hwan LeeJin Koo KimJae Suk HuhJeong Hyun KimIn Seong ChoeTai Wha ChungYong Kyung Choe

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1997

Citation 

Biochemistry and Molecular Biology International, vol. 41, no. 4, pp. 657-663

Keywords 

BCGIsopropylmalate dehydrogenase geneleuBM. tuberculosisbacterial genegenetic complementationmolecular cloningmolecular weightmycobacterium bovismycobacterium tuberculosis

Abstract 

A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1011 bp encoding isopropylmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)