상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Optimization of feeding strategy for overproduction of human lipocortin-I in Saccharomyces cerevisiae controlled by the GAL10 promoter

Authors 

Bong Hyun ChungDong Jin SeoSoo-Wan NamJeong Geol NaYong-Keun Chang

Issue Date 

1997

Citation 

Journal of Fermentation and Bioengineering, vol. 84, no. 5, pp. 466-470

Keywords 

Saccharomyces cerevisiaehuman lipocortin-Ifed-batch

Abstract 

Fed-batch fermentation was conducted to overproduce human lipocortin-I (LC1) in recombinant Saccharomyces cerevisiae controlled by the GAL10 promoter. To optimize the feeding strategy, various fed-batch culture modes were performed with concentrated feed media containing carbon mixtures of galactose (Gal) and glucose (Glu) at three different concentration ratios (Gal: Glu ratios): 9: 1, 1: 1, 1: 9. The cell growth, expression level of LC1, and the plasmid stability were investigated under these fed-batch culture modes. While both glucose and galactose were being fed to the fermentor, the glucose concentration in the culture broth was kept below 1 g/1 for efficient gene expression. High cell concentrations of greater than 100 g dry cell weight/1 were achieved with these fed-batch culture modes. A significant amount of intact LC1 was found to be secreted into the culture medium, but proteolytically cleaved products (des1-26-LC1) were also observed in the culture medium. The fed-batch fermentation with the feed medium at a Gal: Glu ratio of 1: 1 resulted in the highest LC1 total (intact LC1 + des1- 26-LC1) concentration of 500 mg/l, which corresponded to 1.73- and 1.83-fold increases over that produced with the media at Gal: Glu ratios of 1: 9 and 9: 1, respectively.

ISSN 

0385-6380

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)