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Title 

Cloning and phylogenetic analysis of chitin synthase gene from entomopathogenic fungus, Beauveria brongniartii

Authors 

Jin Sik NamDong-Hun LeeHo Yong ParkKyung Sook Bae

Publisher 

Microbiological Society of Korea

Issue Date 

1997

Citation 

Journal of Microbiology, vol. 35, no. 3, pp. 222-227

Keywords 

Beauveria brongniartiichitin synthase genePCRphylogeny

Abstract 

DNA fragments homologous to chitin synthase gene were amplified from the genomic DNA of Beauveria brongniartii by PCR using degenerate primers. Cloning and sequencing of the PCR-amplified fragments led to the identification of a gene, designated BbCHS1. Comparison of the deduced amino acid sequence of BbCHS1 with those of other Euascomycetes revealed that BbCHS1 is a gene for class II chitin synthase. The BlastP search of the deduced amino acid sequence of BbCHS1 displayed the highest rate of similarity, 95.8%, with CHS2 of Metarhizium anisopliae. Phylogenetic analysis of the amino acid sequences confirmed the taxonomic and evolutionary position of B. brongniartii, which was previously derived by traditional fungal classification based on morphological features.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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