상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Isolation, characterization, and phylogenetic position of a new sulfur-oxidizing bacterium

Authors 

So Youn JangJoon Sik YoonYong Kook ShinYong Ha ParkJin Yeol ParkSong Suk YangMoon-Joo KohSeong Myeong YoonJung Sup LeeIn Hwa LeeSi Wouk Kim

Publisher 

Microbiological Society of Korea

Issue Date 

1997

Citation 

Journal of Microbiology, vol. 35, no. 3, pp. 165-171

Keywords 

Facultative chemolithoautotrophsulfur-oxidizing bacteriumThiobacillus

Abstract 

A sulfur-oxidizing bacterium was isolated from mine wastewater and characterized. The isolate was gram-negative, rod (0.2 × 1.2-1.5 μm), nonmotile, catalase positive, and oxidase positive. The optimal pH and temperature for growth were 7.0 and 30°C, respectively. The optimum thiosulfate concentration was 70 mM and the maximum growth rate was 0.081 hr-1. The major ubiquinone contained in the isolate was Q-8. The cellular fatty acid composition was C16:0, C16:1, C18:1, C17cyc, and C19cyc as nonpolar fatty acids, and 3-OH C10:0 and 3-OH C12:0 as hydroxylated fatty acids. The isolate was a facultative chemolithoautotroph which can grow autotrophically on sodium thiosulfate and sodium sulfide and which can grow heterotrophically on yeast extract. It can also grow mixotrophically on sodium thiosulfate and yeast extract. Comparison of the 16S rRNA gene sequence of the isolate with that of Thiobacillus species and Paracoccus thiocyanatus revealed that it is closely related to T. caldus which belongs to the β-subclass of the class Proteobacteria. However, the isolate could not grow at extremely low pH (pH 1-3.5). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolate was tentatively named Thiobacillus sp. strain C.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)