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Title 

Molecular cloning and expression of cDNAs encoding mouse Galβ1,3(4)GlcNAc α2,3-sialyltransferase(mST3Gal III) and Galβ1,4(3)GlcNAc α2,3-sialyltransferase(mST3Gal Ⅳ)

Authors 

Kyoung-Sook KimCheorl Ho KimDeug Yong ShinYoung Choon Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

1997

Citation 

Journal of Biochemistry and Molecular Biology, vol. 30, no. 2, pp. 95-100

Keywords 

cDNA cloningexpressionsialyltransferase

Abstract 

Two kinds of cDNA encoding mouse Galβ1,3(4)GlcNAc α2,3-sialyltransferase (mST3Gal III) and Galβ1,4(3)GlcNAc α2,3-sialyltransferase (mST3Gal IV) were isolated from mouse brain cDNA library by means of a PCR-based approach. The cDNA sequences included an open reading frame coding for proteins of 374 and 333 amino acids, respectively, and the primary structure of these enzymes suggested a putative domain structure consisting of four regions, like that in other glycosyltransferases. The deduced amino acid sequences of mST3Gal III and IV showed a 98% and 89% identity with rat ST3Gal III and human ST3Gal IV, respectively. Northern analysis indicated that the expression of mST3Gal III mRNA was abundant in heart, liver and adult brain, while that of mST3Gal IV mRNA was detected in all tissues tested except for testis, but the level was the highest in liver. Soluble forms of mST3Gal III and IV transiently expressed in COS cells exhibited enzyme activity toward acceptor substrates containing the terminal either Galβ1,3GlcNAc or Galβ1, 4GlcNAc sequences. The substrate preferences of both enzymes were stronger for tetrasaccharides than for disaccharides.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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