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Title 

Medium optimization for production of thermostable alkaline proteases from Bacillus licheniformis NS70

 

Bacillus licheniformis NS70으로부터 내열성 alkaline protease 생산을 위한 배지최적화

Authors 

Ja-Hyup KooIn-Jae ChoiHee-Sop NamHyung-Jae LeeZae-Ik ShinTae Kwang Oh

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1997

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 25, no. 2, pp. 207-211

Keywords 

Medium optimizationProtease productionResponse surface methodology

Abstract 

Media optimization for the production of thermostable protease specifically hydrolyzing defatted soybean meal (DSM) from Bacillus licheniformis NS70 was performed by two methods, one-at-a-time method and response surface methodology (RSM). The best carbon source and nitrogen source for the protease production were lactose and DSM, respectively. The maximum protease production estimated by RSM was 606 U/L at 1.11% lactose and 0.43% DSM, the value of which was nearly consistent to the experimental value of 599 U/L. Yeast extract suppressed the protease production. The medium pH was slightly increased at the beginning stage of fermentation, and it tended to decrease after 8 hours. The optimal pH for the protease production was 7.2 in the batch fermentation.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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