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Title 

The measurement of antifungal effect by MTT assay

 

MTT 방법에 의한 항진균성 활성효과의 측정

Authors 

Dong Gun LeeSung Gu LeeKil Lyong KimKyung Soo Hahm

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1997

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 25, no. 3, pp. 335-337

Keywords 

Amphotericin BAntifungal activityMelittin hydrid peptide

Abstract 

In this study, we show a convenient MTT assay for detect the susceptibility of yeast-like form of Trichosporon beigelii against antifungal agents. This assay was developed based on mitocondrial respiration by determining reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) to formazan. Cells of T. beigelii are seeded into 96-well microtiter plates, and antifungal agents, amphotericin B, magainin and CA-ME hybrid peptide were added with various concentrations. After 24 hr incubation, MTT was added, then incubations were continued for 4 hr. Formazan formation was quantified photometrically after extraction of the formazan with acid sodium dodesyl sulfate (SDS). From this assay, we could obtained MICs of antifungal agents against T. beigelii. The presented method can easily be used as an effective methods to assess the antifungal action of various agents on yeasts with minimal amounts of antifungal agents.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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