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Title 

cDNA sequences for asialoglycoprotein receptor from human fetal liver

Authors 

Dong Gun LeeSung Gu LeeKil Lyong KimKyung Soo Hahm

Publisher 

Springer Verlag (Germany)

Issue Date 

1997

Citation 

Journal of Biochemistry and Molecular Biology, vol. 30, no. 4, pp. 299-301

Keywords 

Hepatitis B virusHepG2MiniexonPolymerase chain reactionpreS1

Abstract 

The asialoglycoprotein receptor (ASGPR) was the first described mammalian lectin that mediates the specific binding and internalization of galactose/N-acetylgalactosamine-terminating glycoproteins by hepatic parenchymal cells. H1 and H2 are known as essential subunits of the functional ASGPR. There were close similarities in ASGPR H2 subunits between cultured cell line HepG2 and normal human liver cells including identical sequences at both termini. It was therefore expected that there may be some similarities between the subunits from normal liver cells and fetal liver cells. The two subunits of human fetal liver ASGPR, designated FL-H1 and FL-H2, were cloned from cDNA library by PCR and the sequences were compared with the known H1 and H2 sequences of HepG2, and the H1 sequence of nomal human liver cells. The results showed that FL-H1 was identical to H1 of HepG2. Whereas FL-H2 contains a 15-bp miniexon, but missing 57-bp at the near upstream from the membrane-spanning domain compared to H2 of HepG2 and normal human liver cells indicating that FL-H2 resulted from a differential splicing compared to HepG2 and normal liver cells.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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