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Title 

The use of multiple antigenic peptide (MAP) in the immunodiagnosis of human immunodeficiency virus infection

Authors 

Song Yub ShinMyung Kyu LeeSun Young KimSo Youn JangKyung Soo Hahm

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1997

Citation 

Biochemistry and Molecular Biology International, vol. 43, no. 4, pp. 713-721

Keywords 

MAPimmunodiagnosisantibody detectionHIV-1 gp41(584-618)HIV-2 gp36(574-602)

Abstract 

In order to develop an ELISA system for the antibody detection of HIV-1 or HIV-2 infections, MAPs for HIV-1 gp41(584-618) and HIV-2 gp36(574-602) corresponding to the immunodominant regions of HIV-1 gp41 and HIV-2 gp36 were used as coating antigens in the ELISA. The MAPs were synthesized by the solid phase method using Fmoc-Lys(Fmoc)-OH and their molecular weights were confirmed by tricine gel electrophoresis. The MAPs reacted with all HIV positive sera (64 samples), but did not react with HIV negative sera (48 samples) obtained from healthy blood donors. The MAPs showed high sensitivity and specificity in anti-HIV 1/2 combo panel and anti-HIV-1 seroconversion panels. The results indicated that the ELISA system using synthetic MAPs of gp41(584-618) and gp36(574-602) as coating antigens can be used as an analytical system for the immunodiagnosis of HIV-1 or HIV-2 infections.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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