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Title 

Limitation of Hu-PBL-scid mouse model in direct application to immunotoxicity assessment

Authors 

Hwan Mook KimSang Bae HanDong Ho HongByung Sun YooGoo Taeg Oh

Publisher 

Elsevier

Issue Date 

1997

Citation 

Journal of Pharmacological and Toxicological Methods, vol. 37, no. 0, pp. 83-89

Keywords 

hu-PBL-scidImmunotoxicity assessmentIgGIgMMitogenicityAntibody production

Abstract 

Hu-PBC-scid mice were directly introduced to the methods of immunotoxicity assessments. Human IgG and IgM was detected 1 week after transplantation. Cyclosporin A (CsA) and cyclophosphamide (CP), which were injected i.p. 4 weeks after transplantation, decreased the serum concentration of IgM after 2-4 days of treatment but not that of IgG. Lymphocyte proliferation induced by various mitogens and primary T-dependent antibody responses to sheep red blood cells could not be measured by using splenocytes of hu-PBL-scid mice. These results were correlated with the fact that human cells were not detected in the spleen, thymus, or blood of hu-PBL-scid mouse but were detected in lymph nodes of the intestine, which were observed by flow cytometric and immunohistochemical examinations. The present results suggest using hu-PBL-scid mice in routine immunotoxicity investigations; lymph nodes of intestines could be used as the lymphocyte sources. In addition, the determination of serum Ig concentration might be used as a experimental item.

ISSN 

1056-8719

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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