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Title 

Genetically engineered human interleukin-6 variant with enhanced stability

Authors 

Sang Chul LeeM J LeeIn Pyo ChoiMyung Hee Yu

Publisher 

Springer Verlag (Germany)

Issue Date 

1997

Citation 

Biotechnology Letters, vol. 19, no. 9, pp. 885-888

Keywords 

recombinant interleukin 6protein stability

Abstract 

A recombinant human interleukin-6 mutant with enhanced conformational stability toward denaturant was obtained by site-specific mutagenesis. The clone was identified as having a single amino acid substitution of Lys70 → Glu. When urea-induced denaturation was monitored by the change in fluorescence intensity at 360 nm, Lys70 → Glu mutation shifted the midpoint of unfolding transition from 5.8 M (wild type) to 6.6 M urea. This mutation did not impair the biological activity.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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