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Title 

A lipopeptide biosurfactant produced by Bacillus subtilis C9 selected through the oil film-collapsing assay

Authors 

Hee Sik KimChang Ho LeeHyun Hyo SuhKeug Hyun AhnHee-Mock OhGi Seok KwonJi Won YangByung Dae Yoon

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1997

Citation 

Journal of Microbiology and Biotechnology, vol. 7, no. 3, pp. 180-188

Keywords 

Bacillus subtilisBiosurfactantLipopeptideOil-film collapsing assay

Abstract 

Bacillus subtilis C9 was selected by measuring the oil film-collapsing activity and produced biosurfactant in a medium containing glucose as a sole carbon source. The biosurfactant emulsified hydrocarbons, vegetable oils and crude oil, and lowered the surface tension of culture broth to 28 dyne/cm. A biosurfactant, C9-BS produced by B. subtilis C9 was purified by ultrafiltration, extraction with chloroform and methanol, adsorption chromatography, and preparative reversed phase HPLC. Structural analyses, IR spectroscopy, FAB mass spectroscopy, amino acid composition, and NMR analyses, demonstrated that C9-BS was a lipopeptide comprising a fatty acid tail and peptide moiety. The lipophilic part consisting of C14 or C15 hydroxy fatty acid was linked to the hydrophilic peptide part, which contained seven amino acids (Glu-Leu-Leu-Val-Asp-Leu-Leu) with a lactone linkage.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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