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Title 

Purification and properties of a thermostable phytase from Bacillus sp. DS11

Authors 

Young Ok KimHyung Kwoun KimKyung Sook BaeJu-Hyun YuTae Kwang Oh

Publisher 

Elsevier

Issue Date 

1998

Citation 

Enzyme and Microbial Technology, vol. 22, no. 1, pp. 2-7

Keywords 

PhytasePhytatethermostable enzymeBucillus sp.

Abstract 

Bacillus species producing a thermostable phytase was isolated from the soil of a Korean cattle shed. An extracellular phytase from Bacillus sp. DS11 was purified to homogeneity by acetone precipitation and Phenyl-Sepharose, Resource S, and Superose 12 column chromatographies. Its molecular weight was estimated to be 44 kDa by SDS-polyacrylamide gel electrophoresis. Its optimum temperature for phytase activity was 70°C and about 50% of its original activity remained after incubation at 90°C for 1O min in the presence of 5 mM CaCl2. Calcium ions were required for thermal stability. The optimum pH for enzyme activity was 7.0 and fairly stable from pH 4.0-8.0. The enzyme had an isoelectric point of 5.3. The K(m) value for phytate was 0.55 mM. Its activity was greatly inhibited by EDTA and metal ions such as Cd2+ and Mn2+. As for substrate specificity it was very specific for phytate and had little or no activity on other phosphate esters. The enzyme efficiently hydrolyzed phytate in rice flour.

ISSN 

0141-0229

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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