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Title 

Increased β-carotene synthesis in Blakeslea trispora under strong alkaline culture condition

Authors 

Seon Won KimWeon Taek SeoYoung Hoon Park

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Biotechnology Letters, vol. 18, no. 11, pp. 1287-1290

Keywords 

beta caroteneculture mediumfungus culturesynthesisBlakeslea trispora

Abstract 

A high yield of β-carotene, 48 mg/l, was obtained by cultivation of a strain of Blakeslea trispora under a strong alkaline culture condition (pH 10), which was significantly higher than those obtained under milder pH conditions (3.26 - 7.80 mg/l at pH 4 to 8). The formation of small pellets at pH 10 was observed, whereas a single large cluster was formed for the pHs ranged from 4 to 8. Span 20 at 0.1 to 1% in the culture medium changed cellular morphology to micro-pellets regardless of the culture pH. The same trend of β-carotene production, depending on the alkalinity of the culture broth, was also observed in the presence of 1% of Span 20 yielding much higher productivity. The amounts of β-carotene yielded at pH 10 and 11 were 92.0 and 100.9 mg/l, respectively, which were about two times higher than those obtained at pH 4 to 9.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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