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Title 

Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae

Authors 

Hyun Ah KangMoon Soo JungWon Kyoung HongJung Hoon SohnEui Sung ChoiSang Ki Rhee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1998

Citation 

Journal of Microbiology and Biotechnology, vol. 8, no. 1, pp. 42-48

Keywords 

Saccharomyces cerevisiaehuman serum albuminsecretionprotein expressionprotein secretion

Abstract 

In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GAL10, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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