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Title 

Secretory expression of human α₁-antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer

Authors 

Bong Hyun ChungSu Jeong KimHyun Ah KangMyeong Hee Yu

Publisher 

Springer Verlag (Germany)

Issue Date 

1998

Citation 

Biotechnology Letters, vol. 20, no. 3, pp. 307-311

Keywords 

alpha 1 antitrypsingalactosegene mutationprotein expressionSaccharomyces cerevisiae

Abstract 

Human α1-antitrypsin (α1-AT) was expressed and secreted in Saccharomyces cerevisiae carrying the gal1 and reg1-501 mutations under the control of GAL10 promoter. Increasing galactose from 0 to 0.5 g/l increased the extracellular concentration of α1-AT, though the secretion efficiency was decreased. The final titre of α1-AT secreted and the secretion efficiency were 17.9 μg/ml and 24.8%, respectively, with 0.5 g galactose/l.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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