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Title 

Characterization and epitope mapping of KI-41, a murine monoclonal antibody specific for the gp41 envelope protein of the human immunodeficiency virus-1

Authors 

Song Yub ShinJung Hyun ParkSo Youn JangMyung Kyu LeeKyung Soo Hahm

Publisher 

Springer Verlag (Germany)

Issue Date 

1998

Citation 

Journal of Biochemistry and Molecular Biology, vol. 31, no. 1, pp. 58-63

Keywords 

HIV-1 gp41(584-599)Monoclonal antibodyMultiple antigenic peptide (MAP)Synthetic peptide

Abstract 

In this study, a mouse monoclonal antibody (mAb) against gp41(584-618), the immunodominant epitope of HIV-1 gp41 envelope protein, was generated. For this purpose, BALB/c mice were immunized with double branched multiple antigenic peptides derived from the HIV-1 gp41(584-618) sequence, and antibody-secreting hybridoma were produced by fusion of mice splenocytes with SP2/0 myeloma cells. One clone producing an antigen specific mAb, termed KI-41 (isotype IgG1) was identified, whose specific reactivity against gp41(584-618) could be confirmed by ELISA and Western blot analysis. Epitope mapping revealed the recognition site of the mAb KI-41 to be located around the sequence RILAVERYLKDQQLLG, which comprises the N-terminal region within the immunized gp41(584-618) peptide. Since this mAb recognizes this specific epitope within the HIV-1 gp41 without any cross-reactivity to other immunodominant regions in the HIV-2 gp36, KI-41 will provide some alternative possibilities in further applications such as the development of indirect or competitive ELISA for specific antibody detection in HIV-1 infection or for other basic researches regarding the role and function of HIV-1 gp41.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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