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Title 

A visual membrane immunoassay for the detection of methamphetamine using an enzyme-labeled tracer derived from methamphetamine and amphetamine

Authors 

Myung Ja ChoiBoris M GorovitsJeong Eun ChoiEun Young SongKyung Soo NamJong Sei Park

Publisher 

Pharmaceutical Society of Japan

Issue Date 

1994

Citation 

Biological & Pharmaceutical Bulletin, vol. 17, no. 7, pp. 875-880

Keywords 

methamphetamineamphetamineimmunogentracer influencevisual membrane immunoassay

Abstract 

A visual membrane enzyme immunoassay is described for the measurement of methamphetamine in urine. To increase assay sensitivity, tracers with chemically similar structures were cross-checked with the antibodies to determine their influence on the antibody binding. Tracers of horseradish peroxidase-labeled methamphetamine (MA-HRP) and amphetamine (A-HRP) derivatives were prepared for this purpose. Significant differences in antibody specificity were found between the two tracers. Based on the results of this study, a pair of an antibody and a tracer was selected and a membrane enzyme immunoassay (EIA) was developed utilizing the competitive binding between methamphetamine and the drug-HRP tracer. UltraBind membrane (0.45 μm) was used as the solid matrix to which the antibody was attached. Using diaminobenzidine substrate with Co2+ ion, a stable grey color appeared on the surface of membrane for MA-negative urine samples. No color appeared for MA-positive urine with a cut-off level of 0.8 ppm.

ISSN 

0918-6158

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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