상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Molecular cloning and the nucleotide sequence of a Bacillus sp. KK-1 β-Xylosidase gene

Authors 

Yong Chin ChunKyung Hwa JungJae Chan LeeSeung Hwan ParkHo Kwon ChungJi Hong Yoon

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1998

Citation 

Journal of Microbiology and Biotechnology, vol. 8, no. 1, pp. 28-33

Keywords 

Bacillusβ-xylosidase genecloningnucleotide sequence

Abstract 

A gene coding for β-xylosidase from thermophilic xylanolytic Bacillus sp. KK-1 was cloned into Escherichia coli using plasmid pBR322. Recombinant plasmid DNAs were isolated from E. coli clones which were capable of hydrolyzing 4-methylumbelliferyl-β-D-xylopyranoside. Restriction analysis showed the DNAs to share a common insert DNA. Xylo-oligosaccharides, including xylotriose, xylotetraose, xylopentaose, and xylobiose were hydrolyzed to form xylose as an end product by cell-free extracts of the E. coli clones, confirming that the cloned gene from strain KK-1 is a β- xylosidase gene. The β-xylosidase gene of strain KK-1 designated as xylB was completely sequenced. The xylB gene consisted of an open reading frame of 1,602 nucleotides encoding a polypeptide of 533 amino acid residues, and a TGA stop codon. The 3' flanking region contained one stem-loop structure which may be involved in transcriptional termination. The deduced amino acid sequence of the KK-1 β-xylosidase was highly homologous to the β- xylosidases of Bacillus subtilis and Bacillus pumilus, but it showed no similarity to a thermostable β-xylosidase from Bacillus stearothermophilus.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)