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Title 

Cloning and expression of a clostridium thermocellum xylanase gene in Escherichia coli

Authors 

Kyung Hwa JungKang Moon LeeHoon KimKi Hong YoonSeung Hwan ParkMoo Young Pack

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1998

Citation 

Biochemistry and Molecular Biology International, vol. 44, no. 2, pp. 283-292

Keywords 

Clostridium thermocellumxylanasecloningxynXexoglucanase

Abstract 

A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was catagorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes a xylanase having the molecular weight of 105 kilodaltons. A number of smaller truncated proteins with activities towards 4-methylumbelliferyl-β-D-cellobioside and xylan were also produced. The enzyme hydrolyzed xylan to xylo-oligosaccharide, indicating typical activity of endo-β-1,4-xylanase. This endoxylanase hydrolyzed carboxymethylcellulose without notable reduction of the viscosity as an exo-β-1,4-glucanase, even though the enzyme exhibited very low levels of activity against other soluble and insoluble cellulosic substrates.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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