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Title 

High-level expression of human insulin-like growth factor II in Escherichia coli

Authors 

Hae Jin RheeYoung Ik LeeKyu-Hwan Yang

Publisher 

Elsevier

Issue Date 

1990

Citation 

Journal of Biotechnology, vol. 13, no. 0, pp. 293-304

Keywords 

Human insulin-like growth factor IIβ-GalactosidaseFusion proteinE. coliEscherichia Coligene expression

Abstract 

A gene encoding mature human insulin-like growth factor II (IGF-II) was constructed from the modified IGF-II cDNA sequence and two double-stranded synthetic oligodeoxynucleotide linkers. It was fused to a truncated lacZ gene such that IGF-II was expressed as part of C-terminus of β-galactosidase. This fused lacZ'-IGF-II gene was under the control of tac promoter and we overproduced the β-galactosidase-IGF-II fusion protein in the Escherichia coli. The fusion protein formed inclusion bodies inside the cells. The fusion protein was purified from the isolated inclusion bodies and IGF-II protein was obtained from their fusion protein by CNBr cleavage. The released IGF-II was confirmed by its molecular weight as determined by SDS-PAGE and by its ability to bind anti-iGF antibody.

ISSN 

0168-1656

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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