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Title 

Staphylococcal methicillin resistance expression under various growth conditions

Authors 

Yoonik LeeHaryoung PooYoung Ik Lee

Publisher 

Microbiological Society of Korea

Issue Date 

1997

Citation 

Journal of Microbiology, vol. 35, no. 2, pp. 103-108

Keywords 

MethicillinStaphylococcuspenicillin binding proteinsdivalent cationspeptidoglycan

Abstract 

To improve the detection of methicillin resistant staphylococci, lowered incubation temperature (30°C) and inclusion of sodium chloride in media have been empirically recommended. However, in this study, we found that sodium chloride in Peptone-Yeast Extract-K2HPO4 (PYK) medium decreased methicillin minimum inhibitory concentrations. Divalent cations were shown to restore the expression of staphylococcal methicillin resistance. However, when it was determined by efficiency of plating, sodium chloride increased methicillin resistance expression on agar medium in which higher divalent cations were contained in the agar medium. The decrease of minimum inhibitory concentrations at 30°C by sodium chloride occurred in Brain Heart Infusion but did not occur in other media investigated. Interestingly, both PYK and Brain Heart Infusion media had peptone, which contain cholic acids having detergent activities. Inclusion of sodium chloride in PYK caused a higher rate of autolysis. Penicillin binding protein 2a that has a low affinity to beta-lactam antibiotics, was highly inducible in methicillin resistant Staphylococcus epidermidis strains. In this study, we found that autolysins that are activated by the sodium chloride decreased the minimum inhibitory concentration at 30°C, and peptidoglycan is weakened due to the presence of methicillin. Peptone in the media may aggravate the fragile cells. However, stabilization due to the presence of divalent cations and production of penicillin binding protein 2a increase the survival of staphylococci.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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