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Title 

Overproduction of human granulocyte-colony stimulating factor fused to the pelB signal peptide in Escherichia coli

Authors 

Bong Hyun ChungMi Jin SohnSu Wan OhUi Sun ParkHaryoung PooBeom Soo KimMi Jin YuYoung Ik Lee

Issue Date 

1998

Citation 

Journal of Fermentation and Bioengineering, vol. 85, no. 4, pp. 443-446

Keywords 

Escherichia colihuman granulocyte-colony stimulating factorhuman granulocytespeptidesgranulocyte colony stimulating factorsignal peptidesignal transduction

Abstract 

In an attempt to induce the secretion of human granulocyte-colony stimulating factor (hG-CSF) in Escherichia coli, the gene encoding mature hG- CSF was fused to the gene for the pectate lyase B (pelB) signal peptide of Erwinia caratovora, and fed-batch fermentation of the recombinant E. coli was performed. No secretion of hG-CSF and no processing of the signal peptide from the fusion protein was observed. Instead, the fusion protein, pelB-hG- CSF, at a concentration of ca. 1.7 g/l was produced in an inclusion body form. Interestingly, the biological activity of the purified fusion protein was found to be identical to that of mature hG-CSF, indicating that the pelB signal peptide attached to the N-terminal of hG-CSF had no effect on its biological activity.

ISSN 

0385-6380

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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