상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

A biotin-avidin labeled enzyme immunoassay for the quantitation of serum TSH using protein-layered solid phase

Authors 

Myung Ja ChoiEun Young SongTai Wha Chung

Publisher 

Pharmaceutical Society of Korea

Issue Date 

1998

Citation 

Archives of Pharmacal Research, vol. 21, no. 3, pp. 231-235

Keywords 

Biotin-avidinEIATSHTrilayered solid phase

Abstract 

A sensitive enzyme immunoassay for serum TSH has been developed utilizing the tight binding between biotin and avidin, and three layered protein polystyrene beads as solid phase. To increase binding capacity of TSH and sensitivity of the assay, the polystyrene beads were coated sequentially with mouse immunoglobulin as first layer, rabbit antimouse immunoglobulin as second layer and monoclonal anti-TSH as third layer. A serum sample was incubated simultaneously with a monoclonal anti-TSH immobilized polystyrene beads and a second monoclonal anti-TSH covalently attached to biotin. After washing, the antibody bound serum TSH-anti-TSH-biotin complex is reacted with horseradish peroxidase (HRP)-labeled avidin. Following a second wash, the bound HRP activity was measured colorimetrically. Reproducible results were obtained within 4 hours for serum TSH in the range between 0 μlU/ml and 50 μlU/ml with detection limit of 0.1 μlU per test.

ISSN 

0253-6269

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)