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Title 

Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as a drug resistance marker for transformation

Authors 

I G KimS K NamJung Hoon SohnSang Ki RheeG H AnS H LeeEui Sung Choi

Publisher 

American Society for Microbiology

Issue Date 

1998

Citation 

Applied and Environmental Microbiology, vol. 64, no. 5, pp. 1947-1949

Keywords 

dna fragmentantibiotic resistanceantioxidant activitybacterium transformationdna determinationgene expressiongene sequencegenetic analysismolecular cloningplasmid

Abstract 

The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/μg of DNA with an integrated copy number of about seven per haploid genome.

ISSN 

0099-2240

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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