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Title | Prolactin-inducible enhancer activity of the first intron of the bovine β-casein gene |
Authors | Yong Kook Kang; Chul Sang Lee; An Sik Chung; Kyung Kwang Lee |
Publisher | Springer Verlag (Germany) |
Issue Date | 1998 |
Citation | Molecules and Cells, vol. 8, no. 3, pp. 259-265 |
Keywords | β-Casein; Enhancer; HC11 Cell; Intron-1; Prolactin |
Abstract | We have analyzed the regulatory roles of the first intron (intron-1) of the bovine β-casein gene in the bovine β-casein/CAT expression system using a mouse mammary epithelial cell line, HC11. After a combined treatment of HC11 cells with insulin, dexamethasone, and prolactin, the induced expression of pβc1.8/+ICAT vector including 2 kb intron-1 and 1.8 kb promoter was greatly increased to 23.5 folds, while that of pβc1.8CAT basic vector with 1.8 kb promoter only, was 6.5. A classical enhancer activity was shown in the 2 kb intron fragment from the experiment in which the orientation and the position of the intron-1 on the vectors were changed. The enhancer activity was largely dependent on the lactogenic hormones, especially prolactin. A stepwise reduction of the inducibility in the 5′ to 3′ deletion analysis of the intron-1 indicates the existence of several functional elements in the region. In particular, an internal fragment (+1071 to +1490) was important for the prolactin-dependent enhancing activity of the intron-1. These results suggest that several elements in the intron-1 of the bovine β-casein gene cooperatively interact not only with each other but also with its promoter for hormonal induction. |
ISSN | 1016-8478 |
Appears in Collections | |
Registered Date |
2019-05-02 |
