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Title 

Rapid and simple purification of biologically active human hepatitis B virus transactivator-X proteins

Authors 

Ha Ryoung PooSun Ok KimMi Jin SohnSook LeeYoung Ik Lee

Publisher 

Microbiological Society of Korea

Issue Date 

1998

Citation 

Journal of Microbiology, vol. 36, no. 1, pp. 55-58

Keywords 

Hepatitis B virus-X proteinexpressionpurification

Abstract 

The human hepatitis B virus-X (HBV-X) was cloned into an expression vector, pET3d, containing a T7 promoter and direct expression was induced in Escherichia coli. The expressed HBV-X protein was purified to homogeneity by centrifugation, ion-exchange chromatography and gel filtration. After gel filtration, renaturation of HBV-X were performed using dialysis against serially diluted urea buffer. The biological activity of refolded HBV-X protein was confirmed by enhancement of protein/DNA complexes using gel-shift analysis.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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