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Title 

Molecular cloning and expression of human α2,8-sialytransferase (hST8Sia V)

Authors 

Yeon Jeong KimKyung Sook KimSu Il DoCheorl Ho KimSe Kwon KimYoung Choon Lee

Publisher 

Elsevier

Issue Date 

1997

Citation 

Biochemical and Biophysical Research Communications, vol. 235, no. 0, pp. 327-330

Keywords 

human tissuemolecular cloningcloning, molecularconserved sequencegene expression regulation, enzymologichumansmolecular sequence data

Abstract 

The cDNA encoding human α2,8-sialyltransferase (hST8Sia V) which exhibits activity toward gangliosides, GM1b, GD1a, GT1b, and GD3, was isolated by screening of human brain cDNA library with a DNA probe generated from the cDNA sequence of mouse ST8Sia V (mST8Sia V) and by 5'-RACE of mRNA from human brain tissue. Comparative analysis of this cDNA with mST8Sia V showed that each sequence of the predicted coding region contains 84% identity in both nucleotide and amino acid. Northern analysis of this cDNA indicated that, in contrast to mST8Sia V, two different sizes of transcripts corresponding to 11 and 2.5 kb were expressed in both human fetal and adult brain, while the transcript of 2.5 kb was detected only in adult heart and skeletal muscle. The enzyme expressed in COS cells showed a substrate specificity very similar to that of mST8Sia V.

ISSN 

0006-291X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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