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Title 

Establishment and characterization of cell lines constitutively expressing hepatitis B virus X-protein

Authors 

Young Ik LeeYong Sik BongHa Ryoung PooYoon Ik LeeJong Gu ParkSu One OhMi Jin SohnSook leeUi Sun ParkNam Soon KimSang Won Hyun

Publisher 

Elsevier

Issue Date 

1998

Citation 

Gene, vol. 207, no. 0, pp. 111-118

Keywords 

Hepatitis B virus-X proteinHepatoma cell lineStable transfectantDNA-binding activityCell cycle analysisHepatocellular carcinoma

Abstract 

We prepared human hepatoma cell lines, which expressed the human hepatitis B virus-X gene product. The plasmid pMAMneo-X, containing an HBV-X gene promoter, an enhancer and a structural gene was constructed. Transfected HBV-X gene integration and expression were detected by Southern and Northern blotting, as well as by chloramphenicol acetylase transferase (CAT) assay using various kinds of promoter-CAT reporter systems. HBV-X protein expression in stable transfectants was confirmed by immunofluorescence microscopy. Transfected cell lines showed permanent expression of HBV-X proteins. The HBV-X transfectant activated its target promoters in promoter-CAT constructs as reporters. The HBV-X transfectant enhanced AP-1 transcription factor binding to its target DNA. Therefore, X-transfectants are not only stable, but also have specific biological functions. Cell cycle analysis by flow cytometry showed that the majority of the transfectant cells are arrested in the G1 or G2 phase of the cell cycle. These cell lines may be useful in analyzing the biological functions of HBV-X and its functional role in the formation of hepatocellular carcinomas.

ISSN 

0378-1119

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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