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Title 

Identification of new GATA factor family genes in Saccharomyces cerevisiae & Schizosaccharomyces pombe

Authors 

Kwang Lae HoeKyung Sook ChungMi Sun WonO J YooHyang Sook Yoo

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1998

Citation 

Biochemistry and Molecular Biology International, vol. 44, no. 5, pp. 897-906

Keywords 

GATA factorzinc fingerS. pombedegenerate PCR

Abstract 

By genomic Southern hybridization using degenerate oligo probes corresponding to the conserved zinc finger of the GATA family, four and three new GATA-factor genes were detected in Saccharomyces cereviaiae and Schizosaccharomyces and pombe respectively. Through degenerate PCR and Southern hybridization using an internal probe between the PCR primers, a new GATA-factor gene of S. pombe, gaf1, was identified. The DNA sequence analysis revealed that the partial gafl gene contains the strictly conserved zinc finger which is the hallmark of the GATA family. As judged form the codon usage of leucine in the zinc finger probe, the zinc fingers of S. pombe are more similar to those of higher fungi than those of S. cerevisiae, and this means that S. pombe is phylogenically close to higher organisms than S. cerevisiae.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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