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Title 

Increment of efficiency in the identification on noble genes by colony hybridization assay

Authors 

Jae Wha KimI A LeeYong Hee LeeJ C SongYong Kyung ChoeY S HahnJ H ChungTai Wha ChungIn Seong Choe

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1998

Citation 

Biochemistry and Molecular Biology International, vol. 44, no. 2, pp. 225-233

Keywords 

Human fetal liver cDNAredundancyprescreeninghybridization assay

Abstract 

For the rapid identification of noble genes in a specific tissue by computer analysis from the cDNA sequences determined by single-pass cDNA sequencing, clone redundancy was one of the major obstacles. To facilitate the efficiency in identification of noble genes, it was necessary to reduce the number of clones to be sequenced by eliminating the redundant clones for a rapid analysis. In order to increase the probability of isolating noble sequences from the cDNA clones of human fetal liver tissue origin, colony hybridization assay was adopted and redundant clones were efficiently removed. Four cDNA clones highly redundant in the human fetal liver cDNA libraries including α-globin, γ-globin, serum albumin and H19 RNA sequences were selected as the probes. Two hundreds and sixty two cDNA clones were randomly selected and tested with the probes for hybridization properties. The identity of each cDNA clone giving positive or negative signals in the hybridization assay was determined by DNA homology search with the nucleic acid databases. Among the 76 clones giving positive signals, 57 clones (75%) were found to be identical to the probe sequences and could be eliminated by colony hybridization assay before neucleotide sequencing.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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