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Title 

Expression of thermostable alkaline protease gene from Thermoactinomyces sp. E79 in E. coli and heat activation of the gene product

Authors 

Jung Kee LeeYoung Ok KimK SunithaTae Kwang Oh

Publisher 

Springer Verlag (Germany)

Issue Date 

1998

Citation 

Biotechnology Letters, vol. 20, no. 9, pp. 837-840

Keywords 

alkaline proteinasegene productEscherichia coligene expressionheatingmolecular cloningthermoactinomycesthermostabilitytime

Abstract 

The expression of Thermoactinomyces sp. E79 protease gene cloned into E. coli was highly host-dependent and the levels of protease expression was most stable in E. coli RR1 and E. coli HB101. Heating the intracellular extract at 70°C for 15 min converted the inactive recombinant E79 protease to its active mature form and also resulted in purification of the enzyme in a single step. Addition of 10 mM CaCl2 to the E79 protease decreased its autolysis and increased its thermal stability.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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