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Title 

Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies

Authors 

Chul Ho KimH W SeoH Y KimJung Hoon SohnEui Sung ChoiSang Ki Rhee

Publisher 

Springer

Issue Date 

1998

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 21, no. 0, pp. 1-5

Keywords 

hirudinHansenula polymorphamethylotrophic yeastmethanol oxidaseautonomously replicating sequence

Abstract 

Various recombinant Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin. H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin. The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined.

ISSN 

0169-4146

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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