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Title 

Rapid method for the determination of lipid from the green alga Botryococcus braunii

Authors 

Seog June LeeByung Dae YoonHee-Mock OH

Publisher 

Springer

Issue Date 

1998

Citation 

Biotechnology Techniques, vol. 12, no. 7, pp. 553-556

Keywords 

lipid analysislipid metabolism

Abstract 

Of various methods for lipid recovery in Botryococcus braunii UTEX 572, the most effective method was disruption of the cells with a bead-beater followed by extraction with chloroform/methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically (r2 = 0.997). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations.

ISSN 

0951-208X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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