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Title 

Effect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae

Authors 

K Janannadha RaoChul Ho KimBong Hyun ChungJung Hoon SohnSang Ki Rhee

Publisher 

Springer

Issue Date 

1998

Citation 

Bioprocess Engineering, vol. 19, no. 5, pp. 385-388

Keywords 

hirudinculture mediumfeeding

Abstract 

Different feeding strategies of galactose were employed to improve the production of anticoagulant, hirudin, by fed-batch mode of cultivation from recombinant Saccharomyces cerevisiae. The structural gene coding for hirudin was harboured with GAL10 promoter for controlled expression of hirudin and the MFα 1 signal sequence for secretion into the growth medium. A stepwise feeding of galactose was found as more suitable feeding strategy of galactose which resulted in the final hirudin volumetric productivity of 6,840 μg/l · h, than intermittent, continuous and ethanol controlled feeding of galactose. The final volumetric productivity of hirudin obtained by step-wise feeding of galactose was 3.88 fold higher compared with simple batch fermentation.

ISSN 

0178-515X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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