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Title 

Identification of a new 5'-noncoding exon region and promoter activity in human N-acetylglucosaminyltransferase III gene

Authors 

Bong Seok KangYeon Jeong KimJae Kyoung ShimEun Young SongYoung Guk ParkYoung Choon LeeKyung Soo NamJune Ki KimTae Kyun LeeTai Wha ChungCheorl Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

1998

Citation 

Journal of Biochemistry and Molecular Biology, vol. 31, no. 6, pp. 578-584

Keywords 

D-N-acetylglucosamine:-D-mannoside-1,4-N-acetylglucosaminyltransferase IIIExpressionPromoter

Abstract 

In a previous paper (Kim et al., 1996a), the immediate 5′-flanking region and coding region of the human UDP-N-acetylglucosamine:-D-mannoside-1,4-N-acetylglucosaminyltransferase III (N-acetylglucosaminyltransferase-III; GnT-III) gene was reported, isolated and analyzed. Herein, we report on amplification of a new 5′-noncoding region of the GnT-III mRNA by single-strand ligation to single-stranded cDNA-PCR (5′-RACE PCR) using poly(A)+ RNA isolated from human fetal liver cells. A cDNA clone was obtained with 5′ sequences (96 bp) that diverged seven nucleotides upstream from the ATG (+1) start codon. A concensus splice junction sequence, TCTCCCGCAG, was found immediately 5′ to the position where the sequences of the cDNA diverged. The result suggested the presence of an intron in the 5′-noncoding region and that the cDNA was an incompletely reverse-transcribed cDNA product derived from an mRNA containing a new noncoding exon. When mRNA expression of GnT-III in various human tissues and cancer cell lines was examined, Northern blot analysis indicated high expression levels of GnT-III in human fetal kidney and brain tissues, as well as for a number of leukemia and lymphoma cancer cell lines. Promoter activities of the 5′-flanking regions of exon 1 and the new noncoding region were measured in a human hepatoma cell line, HepG2, by luciferase assays. The 5′-flanking region of exon 1 was the most active, whilst that of exon 2 was inactive.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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