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Title 

PLCgamma1 Src homology domain induces mitogenesis in quiescent NIH 3T3 fibroblasts

Authors 

Mark R SmithYa Lun LiuSeung Ryul KimYun Soo BaeChan Gill KimKi Sun KwonSue Goo RheeHsiang Fu Kung

Publisher 

Elsevier

Issue Date 

1996

Citation 

Biochemical and Biophysical Research Communications, vol. 222, no. 0, pp. 186-193

Keywords 

cell strain 3t3mitogenesisprotein domainsequence homology3T3 Cellssrc Homology Domains

Abstract 

Previously, we demonstrated that microinjection of phosphoinositide-specific phospholipase Cγy1 (PLCγ1) and lipase-defective mutants of PLCγ1 induced G0 growth arrested NIH 3T3 fibroblasts to enter S phase of the cell cycle. These experiments suggested that regions other than the catalytic domain of PLCγ1 may be responsible for inducing mitogenesis. To test other regions of PLCγ1 for DNA synthesis inducing activity, cDNA fragments encoding Src homology (SH) and pleckstrin homology (PH) domains were subcloned into the bacterial expression plasmid pGEX-2TK, and the GST fusion proteins were purified. The complete PLCγ1 SH domain peptide was found to induce DNA synthesis after microinjection into growth arrested fibroblasts. Peptides containing a single SH3 domain or two SH2 domains induced a partial response that was restored to full activity if they were co-injected. The PH domain peptide did not induce DNA synthesis. Thus, both SH3 and SH2 activity combine to give maximum DNA synthesis induction, demonstrating that non-catalytic structural domains of PLCγ1 have pronounced effects on mitogenic signaling pathways.

ISSN 

0006-291X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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