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Title 

Optimal conditions for the production of immunostimulating polysaccharides from the suspension culture of Angelica gigas cells

 

면역증강성 다당 생산을 위한 참당귀 세포배양의 최적조건

Authors 

Kyung Seop AhnWeon Taek SeoWoong Seop SimIk Hwan Kim

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1998

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 26, no. 2, pp. 130-136

Keywords 

Angelica gigas Nakaiimmunostimulating polysaccharidespolysaccharidessuspension culturepolysaccharide production medium (PPM)

Abstract 

An Immunostimulating polysaccharide was produced from the suspension culture of Angelica gigas H4, plant cells. In order to enhance the polysaccharide production by the A. gigas cell culture, medium composition and physical conditions were optimized. Schenk and Hildebrandt (SH) medium was selected as an optimal basal medium for the growth of A. gigas. The maximum cell and polysaccharide concentration obtained in SH medium were 15.8 g DCW/l and 0.85g polysaccharide/l, respectively, at 25°C under dark condition. For the enhanced polysaccharide production, a polysaccharide production medium (PPM) was established by modifying Gamborg B5 medium with optimized carbon sources, growth regulators, organic and inorganic elements. Optimal initial pH and temperature were 6.0-6.6 and 20°C, respectively, and the dark condition was better than the light condition. The maximum polysaccharide concentration of 1.5 g/l could be obtained through the optimization of the medium composition and physical conditions.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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