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Title 

Characterization of the structural proteins of hepatitis C virus expressed by an adenovirus recombinant

Authors 

Young Rim SeongChan Hee LeeDong Soo Im

Publisher 

Elsevier

Issue Date 

1998

Citation 

Virus Research, vol. 55, no. 0, pp. 177-185

Keywords 

Adenovirus vectorHCVCoreE1E2

Abstract 

Human adenoviruses have been used for mammalian expression vectors and recombinant vaccines for heterologous antigens. We constructed and characterized an infectious adenovirus recombinant containing core-E1-E2 genes of hepatitis C virus (HCV). The core protein was produced mainly during the early phase of viral infection. Expression of HCV E1 and E2 envelope proteins was detected by an immunoprecipitation with HCV-positive patient's sera. The purified E1 and E2 proteins appeared to be composed of mainly a heterodimeric form via noncovalent interaction, as previously observed in other mammalian expression systems. A small portion of E1 and E2 monomers as well as E1E2 aggregates by interdisulfide linkage were detected. Apparently heterodimeric E1E2 complexes were serologically reactive. The results suggest that adenovirus is an useful HCV antigen-expression vector. Copyright (C) 1998 Elsevier Science B.V.

ISSN 

0168-1702

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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