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Title 

Molecular cloning and expression of human galβ1,3galNAc α2,3-Sialyltransferase(hST3Gal II)

Authors 

Y J KimK S KimS H KimC H KimJung Hun KohIn Seong ChoeS TsujiY C Lee

Publisher 

Elsevier

Issue Date 

1996

Citation 

Biochemical and Biophysical Research Communications, vol. 228, no. 2, pp. 324-327

Keywords 

sialyltransferasegene expressionhumanhuman tissuemolecular cloningnonhumanprotein expressioncloning, molecularhumansmolecular sequence data

Abstract 

A cDNA of human Galβ1,3GalNAc α2,3-sialyltransferase (hST3Gal II), which has been known to exhibit much more acceptor substrate preference for glycolipid than for O-linked oligosaccharides of glycoproteins, was isolated from the human liver cDNA library by plaque hybridization using the cDNA of mouse ST3Gal II (mST3Gal II) cloned previously as a probe. Comparative analysis of this cDNA with mST3Gal II indicates 89 and 94% homologies in the nucleotide and amino acid levels, respectively, between the two sequences in the predicted coding region. Northern analysis indicated that the expression of hST3Gal II mRNA is tissue-specific, it being prominent in skeletal muscle and heart, while that in lung and kidney is very low. This enzyme expressed in COS cells showed a similiar activity with that of mST3Gal II.

ISSN 

0006-291X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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